Journal: mBio

Article Title: A Val292 substitution combined with an alanine duplication (ADUP) in the Ω loop of ADC β-lactamase confers reduced susceptibility to advanced β-lactam agents, including cefiderocol

doi: 10.1128/mbio.03518-25

Figure Lengend Snippet: MIC profiles of cefiderocol among ADC variants identified in CRAb clinical isolates. Scatter plot showing the distribution of cefiderocol MICs for each ADC variant. The x -axis represents individual ADC variants, and the y -axis represents the corresponding MIC values (µg/mL) plotted on a log 2 scale. Each dot corresponds to a single isolate. The horizontal line indicates the median MIC value.

Article Snippet: To examine the effects of the V292W substitution in ADC-30 or ADC-33 on cefiderocol resistance, tests were also performed using the Shionogi MIC Dry Plate Cefiderocol (Kyokuto Pharmaceutical Industrial Co., Ltd., Tokyo, Japan), which is commercially available in Japan and was used due to the limited availability of iron-depleted Mueller-Hinton broth.

Techniques: Variant Assay

Journal: Journal of Antimicrobial Chemotherapy

Article Title: Performance of disc diffusion and gradient strip test on different Mueller–Hinton agar media plates and ComASP® and Bruker UMIC ® Cefiderocol Microdilution Panels for cefiderocol susceptibility testing in carbapenem-resistant Pseudomonas aeruginosa

doi: 10.1093/jac/dkaf301

Figure Lengend Snippet: Quality control. Cefiderocol MIC values obtained with the standard BMD, ComASP, UMIC, and by gradient strip test on iron-depleted MH-agar (ID-MH), bioMérieux MH agar (bioMérieux-MH), Liofilchem MH agar (Liofilchem-MH) plates, and disc diffusion (DD) growth inhibition zones on ID-MH agar, bioMérieux-MH agar and Liofilchem-MH agar plates of P. aeruginosa ATCC27853. Replica numbers are on the x -axis and MICs on the y -axis. Green and yellow areas reflect the EUCAST targets and ranges for P. aeruginosa ATCC27853, respectively. μ, mean.

Article Snippet: Cefiderocol gradient strip test was performed using Cefiderocol MTS TM MIC Test Strip (cefiderocol 0.016–256 μg/mL; Liofilchem, Italy), MH agar plates from bioMérieux and Liofilchem, as well as home-made ID-MH agar plates, according to the manufacturer’s instructions ( https://www.liofilchem.com/images/brochure/mic_test_strip_patent/MTS51.pdf ).

Techniques: Control, Stripping Membranes, Diffusion-based Assay, Inhibition

Journal: Journal of Antimicrobial Chemotherapy

Article Title: Performance of disc diffusion and gradient strip test on different Mueller–Hinton agar media plates and ComASP® and Bruker UMIC ® Cefiderocol Microdilution Panels for cefiderocol susceptibility testing in carbapenem-resistant Pseudomonas aeruginosa

doi: 10.1093/jac/dkaf301

Figure Lengend Snippet: Distribution of cefiderocol MICs determined with the reference broth microdilution (BMD) method. The isolates were grouped according to the acquired carbapenem-resistance marker(s). The vertical line denotes the EUCAST clinical breakpoint. MIC readings were performed in accordance with the EUCAST guidance document on BMD testing of cefiderocol.

Article Snippet: Cefiderocol gradient strip test was performed using Cefiderocol MTS TM MIC Test Strip (cefiderocol 0.016–256 μg/mL; Liofilchem, Italy), MH agar plates from bioMérieux and Liofilchem, as well as home-made ID-MH agar plates, according to the manufacturer’s instructions ( https://www.liofilchem.com/images/brochure/mic_test_strip_patent/MTS51.pdf ).

Techniques: Marker